BS-Seq of Chinese alligator, female lung, wild, winter - SRA

SRX10415115: BS-Seq of Chinese alligator, female lung, wild, winter
1 ILLUMINA (Illumina HiSeq 2500) run: 284.5M spots, 64.7G bases, 28.8Gb downloads

Design: The gDNA was spiked with non-methylated DNA fragments for bisulfite conversion quality control purposes. For each sample, 6 mg gDNA and 30 ng DNA were mixed and fragmented into 200300 bp using sonication. After end repair and acetylation, barcodes with methylated cytosines were added to the fragmented DNA. DNA bisulfite conversion was carried out twice using an EZ DNA methylation-GoldTM Kit (Zymo Research, Irvine, CA). The DNA fragments were then amplified by PCR using KAPA Hifi HotStart Uracil+ ReadyMix (Kapa Biosystems, Wilmington, MA). The index-coded samples were clustered using a TruSeq PE Cluster Kit v3-cBot-HS on a cBot Cluster Generation System (Illumina) according to the manufacturers instructions. The BS library was sequenced on the Illumina HiSeq 2500 platform, generating 125-bp paired-end reads.

Submitted by: Zhejiang University

Study: Alligator sinensis methylome of multiple tissues in summer and winter

PRJNA556094 • SRP287401 • All experiments • All runs

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The Chinese alligator, a freshwater crocodilian endemic to China, goes into hibernation in refuges during winter to save energy and survive the harshness of winter. In this study, we sequenced methylome of Chinese alligator of multiple tissues using bisulfte sequencing to comprehensively explore the epigenetic mechanism underlying hibernation and provide new insights into the conservation of Chinses alligator which are being threatened by climate change.

Sample:

SAMN18103741 • SRS8511985 • All experiments • All runs

Organism: Alligator sinensis

Library:

Name: YRDM00281

Instrument: Illumina HiSeq 2500

Strategy: Bisulfite-Seq

Source: GENOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 284.5M spots, 64.7G bases, 28.8Gb

Run	# of Spots	# of Bases	Size	Published
SRR14038849	284,500,530	64.7G	28.8Gb	2021-03-24

ID:: 13733266

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